High-Throughput SARS-CoV-2 and Host Genome Sequencing from Single Nasopharyngeal Swabs
Gorzynski JE, De Jong HN, Amar D, Hughes D, Ioannidis A, Bierman R, Liu D, Tanigawa Y, Kistler AL, Kamm J, Kim J, Cappello L, Neff NF, Rubinacci S, Delaneau O, Shoura MJ, Seo K, Kirillova A, Raja A, Sutton S, Huang C, Sahoo MK, Mallempati KC, Montero-Martin G, Osoegawa K, Watson N, Hammond N, Joshi R, Fernández-Viña MA, Christle JW, Wheeler MT, Febbo P, Farh K, Schroth GP, DeSouza F, Palacios J, Salzman J, Pinsky BA, Rivas MA, Bustamante CD, Ashley EA, Parikh VN (2020). medRxiv 2020.07.27.20163147

Abstract

During COVID19 and other viral pandemics, rapid generation of host and pathogen genomic data is critical to tracking infection and informing therapies. There is an urgent need for efficient approaches to this data generation at scale. We have developed a scalable, high throughput approach to generate high fidelity low pass whole genome and HLA sequencing, viral genomes, and representation of human transcriptome from single nasopharyngeal swabs of COVID19 patients.